In vitro > 항암유효성평가지원센터

신규 항암물질의 in vitro 효능 평가를 통하여 개발 물질의 선별 및 최적화, 적용 암종 선정, 작용기전의 규명, 바이오마커 선정함으로써 다음 단계의 in vivo 동물 실험의 근거를 제시하는 가장 기본적인 시험단계

보유모델

암종

Human

Mouse

Bladder

J82, T24, UMUC3

Brain

SF268, SF295, SF539, SNB19, SNB75, U251

Breast

BT549, HS578T, MCF7, MCF10A, MDA-MB-231, MDA-MB-468, SKBR3, T47D

4T1

4T1-Luc-TdTomato

Cervix

A431, Caski, C33, HeLa, SiHA

Cholangiocarcinoma

SNU1079, SNU1196, SSP-25, TFK1

Colon

Caco-2, COLO205, DLD1, HCT116, HT29, KM12C, LoVo, LS1034, NCI-H716, NCI-H747, SNU769B, SNU1047, SNU1235, SNU-C1, SNU-C2A, SNU-C2B, SNU-C5, SW403, SW480, RKO, WiDr

CT26.WT

MC38

CT26-Luc

MC38-Luc

Esophagus

OE-33

Fibrosarcoma

FSa-II

Glioblastoma

GL26

Head and Neck

CAL27, SCC-9, SCC-15, SCC-25

Kidney

ACHN, A498 CAKI-1, RXF393, SN12C, SN12C-PM6, TK10, UO31, 786-O

Hematologic
Malignancy
Myeloid MOLM-13, MOLM-14, MV4-11, OCI-AML2, THP-1, U937, KG-1, SKM-1, BDCM, HL-60, NB4, K562, MEG-01, KU-812, RPMI-8226 WEHI-3
Lymphoid RCH-ACV, TOM-1, NALM-20, KASUMI-2, CCRF-CEM, JURKAT, MOLT4, DAUDI, RAJI, SR Ba/F3

Liver

Hep3B, HepG2, HepG-Luc-Fluc, Huh7, SK-Hep1, SNU182, SNU398, SNU449

Lung

A549, A549-Luc, Calu-1, Calu-3, EKVX, HCC15, HCC78, HCC95, HCC366, HCC827, HOP62, HOP92, LC-2/Ad, LK-2, NCI-H23, NCI-H69, NCI-H82, NCI-H146, NCI-H187, NCI-H209, NCI-H226, NCI-H322M, NCI-H358, NCI-H441, NCI-H460, NCI-H520, NCI-H522, NCI-H526, NCI-H596, NCI-H647, NCI-H820, NCI-H1299, NCI-H1385 ,NCI-H1395, NCI-H1473, NCI-H1573, NCI-H1581, NCI-H1650, NCI-H1666, NCI-H1703, NCI-H1781, NCI-H1944, NCI-H1975, NCI-H2009, NCI-H2170, NCI-H2228, NCI-H2286, NCI-H3122, PC9

LLC1

LLC1-Luc

Melanoma

A375P, LOXIMVI, MALME3M, M14, MDA-MB-435, SKMEL-2, SKMEL-5, SKMEL-28, UACC-62, UACC-257

B16-F10

Ovary

ES-2-Luc, IGROV1, NCI/ADR-RES, OVCAR3, OVCAR3-Luc, OVCAR5, OVCAR8, SKOV3, SKOV3-Luc, SNU8, SNU251, UWB1.289 + BRCA1 null, UWB1.289 + BRCA1 overexpression

ID8

Pancreas

AsPC-1, AsPC-1-Luc, BxPC-3, BxPC-3-Luc, MiaPaCa2, PANC-1

PAN 02

Prostate

DU145, LNCaP, PC3, Vcap, 22RV1

TRAMP-C1

Stomach

OE19, SNU1, SNU5, NCI-N87

서비스 내용

  • In vitro drug efficacy test : Cell viability / Death assay / IC50 calculation / Synergism analysis
  • In vitro tumorigenic potential test : Clonogenic assay
  • Signal pathway analysis : Immuno blotting, Immunoprecipitation, ELISA, Q-PCR, Chromatin Immunoprecipitation, Transfection, Protein binding assay
  • Enzyme assay : Kinase assay, Methyltransferase assay
  • Immunocytochemistry, TUNEL staining, Immunofluorescence staining, Hypoxia staining, Pimonidazole, ROS staining, Calcium/zinc ion staining, MitoTracker/sensor staining, LysoTracker/sensor staining, Autophagic vacuole staining
  • MOA 검증

서비스 예시 (IC50 calculation)

  • 세포주에서 Combination Index (CI)
    산출을 통한 병용효과 확인

  • Enzyme assay 및 IC50 산출

  • In vitro 세포 생존율 측정 및 IC50 산출